SubtiBank SubtiBank
lipA [2018-01-03 16:26:52]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

lipA [2018-01-03 16:26:52]

trigger enzyme: lipoic acid synthase and regulator of comE operon expression
locus
BSU32330
pI
8.25
mw
33.77 kDa
protein length
298 aa Sequence Blast
gene length
894 bp Sequence Blast
function
lipid metabolism, required for the synthesis of branched-chain amino acids
product
trigger enzyme: lipoic acid synthase
essential
no
ec
2.8.1.8
synonyms
yutB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.5|Trigger enzymes that control transcription in a yet unknown way]

    • Gene

    Coordinates
    3,320,324 → 3,321,220

    Phenotypes of a mutant

  • reduced transformation efficiency [Pubmed|19028902]
  • strong inhibition of growth on minimal media [Pubmed|19820084]

    • The protein

    Catalyzed reaction/ biological activity

  • Protein N(6)-(octanoyl)lysine 2 sulfur 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine 2 L-methionine 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol H2O = diacylglycerol a carboxylate (according to Swiss-Prot) required for ''[gene|788725411B2E741103603373E43441FD036E1BA0|comEA]'' transcription [Pubmed|19028902]

    • Protein family

  • Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)

    • Structure

  • [PDB|4U0O] (from ''Thermosynechococcus elongatus'', 48% identity) [Pubmed|25100160]

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|2EC820F54911603A99444DC922E41C60AA526EB2|lipA]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B578 (yutB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1577 NBRP B. subtilis, Japan]
  • BKE32330 (Δ[gene|2EC820F54911603A99444DC922E41C60AA526EB2|lipA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCCCATCATCTCCAAT, downstream forward: _UP4_TAATGCCAAAACGCCAGATC
  • BKK32330 (Δ[gene|2EC820F54911603A99444DC922E41C60AA526EB2|lipA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCCCATCATCTCCAAT, downstream forward: _UP4_TAATGCCAAAACGCCAGATC

    • References

    Reviews

  • 27074917

    • Original publications

  • 18957862,19028902,19820084,25100160